Does John Holt have a Wikitree profile? If not, I encourage you to create a profile and add the research you have gathered.
There isn’t enough infomation posted on Jacob’s profile to answer your question. John was not mentioned in Jacob’s Last Will and Testament. However, not all heirs were named in the document. Other probate records for him or his wife may reveal more.
https://www.wikitree.com/wiki/Holt-8277