Thanks for pointing this out, as I always had this connection on my tree, and since so many others also did, I never really questioned it until now. However, what is interesting, is there's a blog about a whole romantic story about how Lady Jane Cooper was the 1st cousin of John and William Ashley, and that the two brothers were both in love with her, and that they decided whoever she picked, the other would move to the colonies, so John won her hear, and William went to the colonies, and then John and Jane then followed, and went to the colonies. See [
http://skyvington.blogspot.com/2009/02/lord-and-his-lady.html] There are also two rivers, called Cooper River and Ashley river, after the 1st earl of Shaftesbury. See [p. 325 of, "The South Carolina Historical and Genealogical Magazine, Volume 1"] Also, I should point out, that the lack of evidence of Lady Jane ever being mentioned in any of the above sources as another daughter, doesn't necessarily mean she didn't exist as their daughter, as a negative doesn't prove lack of connection. And the sources consulted appear to be sources were baronetcy books, and females wouldn't necessarily be as relevant in those, since they wouldn't inherit the baronetcy, and Wikipedia is just people putting things in, and there are mistakes all the time, as I often comment on talk pages when I see such errors, which is more frequent than I would like. Additionally, the birth year we have for Jane is 1627, and if Anne Ashley died in 1628 of small pox, then she would have only been a baby. The children were sent to live with other guardians, namely a Sir Daniel Norton, and then their maternal uncle, Sir Edward Tooker. So possibly the daughter got shuffled to a different household, and so history has not recognized her as much, or maybe was forgotten. I can only speculate. Also, my family line, shows that Jane Cooper's granddaughter, another Ann Ashley, b. 1701, married a Sir James Forrest, whose son William, married into the Gresham family, who we have showing as being great nobility as well, and includes the Lennards as ancestors, who once were the estate keepers of one of the largest manor houses in England, Knole Park. Also, several generations up one of the lines, we have an Elizabeth Monck on the Gresham family. Interestingly, the Earl of Shaftesbury was very politically involved with the Monck's, and supported Sir George Monck's motivations to restore the Rump Parliament (what was remaining of the legitimate parliament from before Cromwell dissolved the parliament after dethroning Charles II). Anyways, very interesting, and clearly there are weaknesses in the connection here, so I think it is worth investigating, as I would like to know where my family line truly goes.