Maria (29 June 1482 – 7 March 1517), a daughter of Queen Isabella I of Castilla and King Fernando II. She was the fourth of five surviving children and had a twin sister, Anna, who was stillborn.
Infante Afonso (23 April 1509 - 21 April 1540) Cardinal of the Kingdom.
King Henry (31 January 1512 -31 January 1580) Cardinal of the Kingdom who succeeded his grandnephew King Sebastian (Manuel's great-grandson) as 17th #King of Portugal. His death triggered the struggle for the throne of Portugal.
e.g., Calendar State Papers Spain, vol. 1 (1862) no. 210, Pedro de Ayala to Ferdinand and Isabella, 25 July 1498.
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